Applications of Biotechnology Chapter Quiz

Applications of Biotechnology Chapter Quiz

Group 5

Format

• Answer the question in the time limit

• There is a short pause after each question to explain if needed and look at the results

• The time limit is 20 seconds

• Good Luck!!

Multiple Choice

The Ti plasmid used for producing transgenic plants is found in

• Azotobacter

• Rhizobium

• Azospirillum

• Agrobacterium

Multiple Choice

a-1 antitrypsin is:

• an antacid

• an enzyme

• used to treat arthritis

• used to treat emphysema.

Fill in the Blanks

The site of production of ADA in the body is

.

Answers- Lymphocyte/Bone marrow

Lymphocytes are kind of white blood cells

present in the bone marrow.

ADA (adenosine deaminase) is an enzyme that is present in lymphocytes and is very important for the immune system to function.

 Its primary function in humans is the development and maintenance of the immune system.

Multiple Choice

Which among the following is based on antigen-antibody interaction?

• PCR

• Electrophoresis

• ELISA

• All of the above

Multiple Choice

C-peptide of human insulin is

• a part of mature insulin molecule

• responsible for formation of disulphide bridges

• removed during maturation of pro-insulin to insulin

• responsible for its biological activity.

Explanation

Pro hormones needs to be processed before it becomes a maturation into a functional hormone. In pro insulin there is an extra stretch c- peptide which needs to be removed during maturation .

Multiple Choice

In RNAi (RNA interference), genes are silenced using

• ss DNA

• ds DNA

• ds RNA

• ss RNA

Explanation

ds RNA is a double stranded RNA. So using the agrobacterium vector, nematode specific genes were introduced into the host cell which caused the DNA to produce both sense and antisense RNA which were complimentary therefore forms dsRNA which silences the mRNA of the nematode and the parasite could not survive in a transgenic host expressing RNAi.

Multiple Choice

What does GEAC Stand for

• Genetic Engineering Approval Committee

• Genetic Engineering Advisory Council

• Genetic Export Approval Committee

• Global Environmental Advisory Council

Fill in the Blanks

Which part of the tobacco plant is infected by Meloidegyne incognitia?

Multiple Choice

Some of the characteristics of Bt cotton are

• long fibre and resistance to aphids

• medium yields, long fibres and resistance to beetle pests

• high yield and production of toxic protein crystals which kill dipteran pests

• high yield and resistance to bollworms

Multiple Choice

ELISA involves

• Using radiolabelled second antibody

• Using complement-mediated cell lysis

• Usage of RBCs

• Addition of substrate that is converted into a coloured end product

Explanation

In ELISA, antigens from the sample are attached

to a surface. Then, a matching antibody is applied over the surface so

it can bind to the antigen. This antibody is linked to an enzyme, and in

the final step, a substance containing the enzyme's substrate is

added. The reaction produces a detectable signal, a colour

change. Hence, the statements can be related to ELISA is that It

involves the addition of substrate which is converted into the coloured

end product.

Multiple Choice

RNA interference mainly helps in

• Cell proliferation

• Micropropagation

• Cell defence

• Cell differentiation

Multiple Choice

The expression of a transgene in the target tissue is identified by a

• Transgene

• Promoter

• Enhancer

• Reporter

Multiple Choice

Assertion : "Rosie", the first transgenic cow, produced human protein‐enriched milk which is more balanced product for human babies than natural cow milk.

Reason : The milk contained the human alpha‐ (α ) lactalbumin

• Both assertion and reason are true and reason is the correct explanation of assertion

• Both the assertion and reason are true but reason is not the correct explanation of assertion

• Assertion is true but reason is false

• Reason is true but Assertion is false

• Both are false

Multiple Choice

Human insulin is being commercially produced from a transgenic species of

• Escherichia

• Mycobacterium

• Rhizobium

• Saccharomyces

Multiple Choice

An improved variety of transgenic basmati rice

• Does not require chemical fertilizers and hormones

• Gives high yield and is rich in Vitamin A

• Gives high yield and has no characteristic aroma

• Is completely resistant to all insect pests and diseases of paddy

Multiple Choice

The trigger for activation of toxin of Bacillus thuringiensis is

• acidic pH of stomach

• high temperature

• alkaline pH of gut

• mechanical action in the insect gut

Multiple Choice

The genes crylAb and cryllAb produce toxins against _______ and _______, respectively.

• cotton bollworms, corn borer

• nematode, cotton bollworm

• corn borer, nematodes

• corn borer, cotton bollworm

Multiple Choice

Match the following

• A-2,B-3,C-4,D-5,E-1

• A-1,B-2,C-4,D-5,E-3

• A-3,B-2,C-4,D-1,E-5

• A-2, B-3, C-4, D-1, E-5