BIOL 3314 Exam 1

BIOL 3314 Exam 1

1. Warm-up

2. Interactive presentation

3. build your review

Poll

• Tampa Bay Buccaneers

• Kansas City Chiefs

Poll

• Team Kong

• Team Godzilla

Poll

• Early Bird

• Night Owl

• exhausted pigeon

Poll

• coffee

• tea

• other

Poll

• Dog person

• Cat person

Poll

Who wore it better?

• Left

• Right

Poll

Pineapple on pizza?

• Yes please!

• What is wrong with you?

Poll

How prepared do you feel for the exam?

• Well prepared

• somewhat prepared

• I need to review more

The Central Dogma of Life

genotype to phenotype

Multiple Choice

Which of the following is NOT one of the main components of the Cell Theory?

• All biological organisms are composed of cells

• Cells are the unit of life

• New cells are produced from pre-existing cells

• Cells must contain DNA

Multiple Choice

This picture depicts a

• nucleoside

• nucleotide

• amino acid

• protein

DNA Replication

*where does it start?

*what mediates it?

*what is the result?

Multiple Select

Which of these are true of ALL DNA polymerase (several are correct)

• synthesize DNA only in the 5’ to 3’ direction

• adding a dNTP to the 3’ hydroxyl group of a growing chain

• are not able to start de novo, meaning they require a primer

Multiple Choice

We know helicase opens up the DNA strands, what would be the result of Helicase not working correctly?

• immediate stop of replication

• slow stop replication

• non-stop replication

Multiple Choice

The enzymes that assure high processivity replication by assuring DNA polymerase remains attached to the DNA are the

• sliding clamp proteins

• SSB proteins

• Topoisomerases

• Telomerases

Multiple Select

The short fragments of DNA produced

during DNA replication are joined together by

• RNase H

• ligase

• DNA polymerase

• single stranded binding proteins

Transcription

*where does it start?

*what mediates it?

*what is the result?

Multiple Choice

The first step to initiate transcription is binding to the TATA box, which is done by what factor?

• TFIIB

• TFIID

• TFIIH

• TFIIE

"Do Bring Harry"

"Do your Best Honey"

TFIID-binding to TATA

TFIIB-brings polymerase

TFIIH-magic of 2 enzymes

Multiple Choice

Where is the 3' end of this new synthesized RNA?

• A

• B

• C

• D

Multiple Choice

Release of RNA Polymerase II to transition from initiation to elongation occurs thanks to

• TFIIB

• TFIID

• TFIIH helicase activity

• TFIIH kinase activity

Multiple Choice

The same Helicase that mediates the opening of the DNA in Replication is used in Transcription

• True

• False

RNA Processing

*splicing

*5'cap

*3'polyAtail

Multiple Choice

The spliceosome makes these structures into lariats or loops and removes them from the pre-mRNA

• exons

• introns

• 5'cap

• 3'poly A tail

Multiple Select

What are the purposes of RNA processing? (several options may be correct)

• form mature mRNA that can leave the nucleus

• recognition for the ribosome

• shorten the RNA chain

Translation

*where does it start?

*what mediates it?

*what is the result?

Multiple Choice

Translation is mediated by

• DNA polymerase

• RNA polymerase

• the ribosome

• mRNA "messenger" or template

• tRNA "transfer" brings the aa

• rRNA "ribosomal"

Things to be on the look out for...

• Consumption of energy

• Start and stop codons

• charged and activated tRNA

You got this!

• I'm always rooting for you, please email me if you have any questions! Have a wonderful day.

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