BIO111 Chp 14 DNA

BIO111 DNA

Chapter 14

Multiple Choice

What did Griffith's "Transforming Principle" experiment conclude to?

• DNA replication was semiconservative

• DNA was the genetic material

• Something (DNA) was transferred from the dead bacteria to the live bacteria

Multiple Choice

Oswald, Macleod, and McCarty broke down the heat-killed S strain and isolated the parts to conclude that genetic information was most likely made of what?

• Proteins

• Lipids

• Nucleic acids

• Carbohydrates

Multiple Choice

Hershey and Chase blender experiment gave evidence of what?

• DNA replication was semiconservative

• DNA was the genetic material

• Something (DNA) was transferred from the dead bacteria to the live bacteria

Multiple Choice

Before Hershey and Chase blender experiment, what did most people believe the genetic material was made of?

• Proteins

• Nucleic acids

• Lipids

• Carbohydrates

Fill in the Blanks

Is DNA double or single stranded? (1 word)

Fill in the Blanks

DNA strands run in opposite directions, otherwise known as

Multiple Choice

Meselson and Stahl's experiment gave evidence of what?

• DNA replication is semiconservative

• DNA is the genetic material

• Something (DNA) was transferred from the dead bacteria to the live bacteria

Multiple Choice

Which enzyme unzips the DNA for replication?

• DNA polymerase

• Helicase

• Topoisomerase

• RNA Primase

Multiple Choice

Which enzyme keeps the DNA from supercoiling after helicase unravels it?

• DNA Polymerase

• RNA Primase

• Topoisomerase

• Single stranded binding proteins

Multiple Choice

Which enzyme is responsible for synthesizing the new DNA strand?

• Ligase

• Single stranded binding proteins

• RNA Primase

• DNA Polymerase

Multiple Choice

Which enzyme is responsible for laying down the RNA primer for DNA Polymerase to bind to?

• RNA Primase

• Helicase

• Single stranded binding proteins

• Topoisomerase

Multiple Choice

Which enzyme is responsible for keeping the DNA strands separated after helicase unzips it?

• DNA Polymerase

• Single stranded binding proteins

• Topoisomerase

• Ligase

Multiple Choice

Which enzyme is responsible for annealing the okazaki fragments together to form a single DNA molecule?

• Primase

• Helicase

• Ligase

• DNA Polymerase

Multiple Choice

DNA Replication (shortens or lengthens) your telomeres by working with telomerase over your lifespan?

• Shortens

• Lengthens

Multiple Choice

Which type of mutation has occurred when a substitution that does not end up changing the amino acid sequence?

• Missense

• Silent

• Nonsense

• Frameshift

Multiple Choice

Which type of mutation has occurred when a substitution caused the amino acid to change into a stop codon?

• Missense

• Silent

• Nonsense

• Frameshift

Multiple Choice

Which type of mutation has occurred when there was an insertion or deletion of a nucleotide?

• Missense

• Silent

• Nonsense

• Frameshift

Multiple Choice

Which type of mutation has occurred when a substitution causes a change in the amino acid sequence?

• Missense

• Silent

• Nonsense

• Frameshift

Multiple Choice

What does Sanger sequencing do?

• Separates pieces of DNA based on size in an electrode

• Sequences DNA with the use of many pieces of DNA cut up by various dideoxynucleotides

• Uses computer imaging to get a photo of chromosome size/shape/banding

Multiple Choice

What does Gel Electrophoresis do?

• Separates bands of DNA based on size through an electrode

• Sequences DNA with the use of many pieces of DNA cut up by various dideoxynucleotides

• Uses computer imaging to get a photo of chromosome size/shape/banding

Summary/What to Study

Griffeth Experiment, Hershey and Chase Experiment, Meselson and Stahl Experiment, DNA Replication process, DNA Replication proofreading, Telomerase action, DNA mutations, Sanger Sequencing, Gel Electrophoresis