Activity 1

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STO

Mutated DNA Sequence #1    TAC    ATC    TTG   GCG   ACG   ACT

What’s the mRNA sequence?

 

• AUG  UGG   AAC    CGC   UGC  UGA

• AUG  UAG   AAC    CGC   UGC  UGA

• ATG  TGG   AAC    CGC   TGC  TGA

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence #1    TAC    ATC    TTG   GCG   ACG   ACT

                                mRNA       AUG  UAG    AAC    CGC   UGC   UGA

What will be the amino acid sequence?

• Met- Trp – Asn-    Arg    Cys -  STOP

• Met- STOP – Asn-    Arg    Cys -  STOP

• Met- STOP

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence #1    TAC    ATC    TTG   GCG   ACG   ACT

                                mRNA       AUG  UAG    AAC    CGC   UGC   UGA

Will there be an effect?

• YES

• NO

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence #1    TAC    ATC    TTG   GCG   ACG   ACT

                                mRNA       AUG  UAG    AAC    CGC   UGC   UGA

        Met  Stop

What kind of mutation is this?

• Substitution- Non sense

• Substitution- MIs sense

• Substitution- Silent

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence # 2     TAC    GAC    CTT   GGC   GAC   GAC T

                             What’s the mRNA sequence?

• AUG CUG GAA CCG CUG CUG A

• AUG  UGG   AAC    CGC   UGC  UGA

• AUG UGG GAA CCG CUG CUG A

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence # 2     TAC    GAC    CTT   GGC   GAC   GAC T

                         mRNA AUG CUG GAA CCG CUG CUG A

What will be the amino acid sequence?

• Met- Trp – Asn-    Arg    Cys -  STOP

• Met-Leu-Glu-Pro -Leu-Leu

• Met-Leu-Asn- Arg    Cys -  STOP

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence # 2     TAC    GAC    CTT   GGC   GAC   GAC T

                         mRNA AUG CUG GAA CCG CUG CUG A

Will there be an effect?

• YES

• NO

Multiple Choice

Original DNA Sequence    TAC    ACC    TTG   GCG   ACG   ACT

mRNA Sequence                AUG  UGG   AAC    CGC   UGC  UGA   

Amino Acid Sequence       Met- Trp – Asn-    Arg    Cys -  STOP

Mutated DNA Sequence # 2     TAC    GAC    CTT   GGC   GAC   GAC T

                         mRNA AUG CUG GAA CCG CUG CUG A

What kind of mutation is this?

• Frameshift-Insertion

• Frameshift -Deletion

• Point Substitution-Missense