DNA Replication

​DNA Replication

Enzymes​ and Replication Steps
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Match

Match the following

• Cell increases in size, new organelles produced

• Cell's DNA is replicated

• Organelles replicated, cell continues to grow

• Cell's nucleus divides

• Cell's cytoplasm divides

G1 phase

S phase

G2 phase

Mitsosis

Cytokinesis

• G1 phase

• S phase

• G2 phase

• Mitsosis

• Cytokinesis

Multiple Choice

When does the cell’s DNA replicate?

• G1 phase

• S phase

• G2 phase

• M phase

Match

Pair the following using the Base Pairing Rule

• A

• C

• T

• G

T

G

A

C

• T

• G

• A

• C

Match

Pair the following using the Base Pairing Rule

• Adenine

• Cytosine

• Thymine

• Guanine

Thymine

Guanine

Adenine

Cytosine

• Thymine

• Guanine

• Adenine

• Cytosine

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​Replication assures that every cell has a complete set of identical genetic

information.

Multiple Choice

DNA replication is described as ________ when new DNA is composed of one strand from the parent DNA and one strand that is newly synthesized.

• semi-conservative
• semi-permeable

• semi-solid

• semi-colon

Match

Match the following: Enzymes to Functions

• Helicase

• DNA Polymerase

• Ligase

• Primase

enzyme unzips DNA like a zipper, creating two single strands of DNA

Builder enzyme that synthesizes new strands of DNA by adding DNA nucleotides

Enzyme that joins or glues fragments of new DNA together

Enzyme that creates a starting point for DNA polymerase

• enzyme unzips DNA like a zipper, creating two single strands of DNA

• Builder enzyme that synthesizes new strands of DNA by adding DNA nucleotides

• Enzyme that joins or glues fragments of new DNA together

• Enzyme that creates a starting point for DNA polymerase

Fill in the Blanks

What is the complementary base pairing for the following sequence?

A A T T G G C C

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Explain the enzymes and steps of DNA Replication​

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​

​Enzyme - Helicase unzips double-stranded DNA helix by breaking hydrogen bonds between complementary base pairs, separating into single strands

​

​Enzyme - Primase attaches RNA primers to DNA, starting point for DNA polymerase

​

​SSB proteins attach to unzipped DNA, stops from joining back together.

Topoisomerase relaxes DNA helix​ from super-coiled structure

​

​DNA polymerase builds new DNA strands by adding new nucleotides. Can only build in the 5′ to 3′ direction. Creating a leading and lagging strand

​

​Enzyme - Ligase joins or glues DNA fragments together

​

​Exonuclease removes primers, they are made of RNA. DNA polymerase replaces them with DNA nucleotides

​

​Second type of DNA polymerase scans DNA for any errors and fixes any found

Fill in the Blanks

What is the complementary base pairing for the following sequence?

A T A T C G C G

Match

Match the following: Steps of DNA Replication

• 1. Unzipping

• 2. Stabilizing

• 3. Priming

• 4. Building and Extending

Helicase unzips DNA helix

SSB proteins prevent DNA from binding back together

Primase marks starting point for DNA polymerase

DNA polymerase builds new DNA strands in 5' to 3' direction

• Helicase unzips DNA helix

• SSB proteins prevent DNA from binding back together

• Primase marks starting point for DNA polymerase

• DNA polymerase builds new DNA strands in 5' to 3' direction

Match

Match the following: Steps of DNA Replication

• 6. Gluing

• 5. Replacing

• 7. Proofreading

Ligase joins DNA fragments together

Exonuclease removes primers, DNA polymerase replaces with nucleotides

DNA polymerase scans DNA for errors, fixes any found

• Ligase joins DNA fragments together

• Exonuclease removes primers, DNA polymerase replaces with nucleotides

• DNA polymerase scans DNA for errors, fixes any found

​Know the enzymes and steps of DNA replication

​I Will...

Explain the enzymes and steps of DNA Replication​

​We Will...

​Win for the Day...