BACE Complex Solutions

Complex Solutions

Because Biology is complicated...

Complex Solutions Have Many Ingredients

First figure out the final volume you will need, or V₂
Address each component individually.
Decide how to best prepare the solution logically.
Get very close to the final volume before adjusting pH (if needed).

​Sample Problem

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


1. To make 500mL of 0.01M Tris-HCl:

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


1. To make 500mL of 0.01M Tris-HCl:

500mL x (1L/1000mL) x (0.1 mol/1 L) x (157.6g/1 mol) =

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


1. To make 500mL of 0.01M Tris-HCl:

500mL x (1L/1000mL) x (0.1 mol/1 L) x (157.6g/1 mol) = 78.8g Tris-HCl

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


2. To make 500mL of 0.25M glucose:

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


2. To make 500mL of 0.25M glucose:

500mL x (1L/1000mL) x (0.25mol/1L) x (180g/1mol) =

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


2. To make 500mL of 0.25M glucose:

C1V1 = C2V2

(3M)(V1) = (0.25M)(500mL)

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


2. To make 500mL of 0.25M glucose:

C1V1 = C2V2

(3M)(V1) = (0.25M)(500mL)
V1 = (0.25 x 500) / 3
V1 = 41.7mL glucose stock solution

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


3. To make 0.1% (vol/vol) Tween-Twenty:

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


3. To make 0.1% (vol/vol) Tween-Twenty:

500mL x (0.1mL/100mL) =

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


3. To make 0.1% (vol/vol) Tween-Twenty:

500mL solution x (0.1mL Tween/100mL solution) = 0.5mL Tween

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


4. To make 5% (wt/vol) BSA:

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


4. To make 5% (wt/vol) BSA:

500mL solution x (5g BSA /100mL solution) =

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


4. To make 5% (wt/vol) BSA:

500mL solution x (5g BSA /100mL solution) = 25g BSA

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.

You have determined you will need the following ingredients:

78.8g Tris-HCl (powder)
41.7mL of glucose (liquid)
0.5mL Tween (liquid)
25g BSA (solid)

Now consider how to combine them.

You want to make 500mL of 0.01M Tris-HCl, 0.25M glucose, 0.1% (vol/vol) Tween-Twenty, 5% (wt/vol) BSA.

You have stock of powdered Tris-HCl (MW 157.6 Da), 3M glucose stock, ample Tween-20 liquid, and solid BSA.


Preparation:
Dissolve the solid Tris-HCl in about 70% of the water you will need (350mL).
Then dissolve the BSA. (Order doesn't really matter here.)
Add the liquid glucose and Tween while stirring. (Maybe you have a stir bar going.)
Raise the water level to near the 500mL total.
Check and adjust pH.
Finalize the water addition to reach 500mL final volume.