BACE Lab Safety & PPE F24

General Lab Safety & PPE

aka Please be safe in the lab because if you aren't, you or someone else could get hurt and/or the work could be ruined, etc.

Multiple Choice

Which of the following is of least concern regarding safety hazards in the biological science laboratory?

• chemicals

• biohazards

• fire

• sharps

• trips & falls

Training before use
Awareness and updated knowledge
SOPs
Cooperation and Teamwork
Reporting and Documentation

Preventative Measures

Chemicals
Biohazards
Electrical & Fire Hazards
Sharps, broken glass, etc.
Equipment misuse

Common Hazards

Basic Laboratory Safety

​Personal Protective Equipment (PPE)

Items worn to protect the user against specific hazards
Often protect eyes, face, clothing, shoes, skin, hearing, lungs, etc.

Multiple Choice

Choose the eyewear you'd most likely wear when inoculating solid media with bacterial cultures.

Multiple Choice

Choose the eyewear you'd most likely wear when preparing solutions for use in the lab.

Multiple Choice

Choose the eyewear you'd most likely wear when viewing an SDS-PAGE gel in which you separated native and denatured GFP.

​Basic PPE Considerations

Choose gloves fit to the purpose
Choose lab coat, apron, or jumpsuit as needed
Have heat- or cold- resistant gloves at the ready
Sometimes you need shoe protection too

Reorder

Place the glove types in order, from least protective to most protective.

• plastic

• thin nitrile

• thick nitrile

• rubber

• neoprene

1

2

3

4

5

• 1
• 2
• 3
• 4
• 5

Multiple Choice

Which of the following is NOT an advantage of nitrile gloves over latex gloves?

• less chance of allergic reaction

• rips and punctures are easier to see

• less expensive

• equal short-term resistance to chemicals

• provide protection from heat and cold

Respiratory Protection

Surgical-type masks offer protection from low levels of microorganisms, dust, and some environmental contaminants

Respirators filter small particles from the air; rated by smallest size excluded
In science labs, most are "filtering face-piece respirators" such as N95s
Labs working with pathogens may use "supplied air respirators" that use air hoses attached to a full containment suit

​Choose PPE Wisely

Balance safety with freedom of movement
Know what you are working with and plan ahead
When in doubt, go with the safer option
Rip, tear, puncture, splash? Replace the PPE asap.

Cleanrooms

Used in Biotech and Pharma to prevent contamination of products
Tightly controlled temp, humidity, pressure
Air flow is regulated to control contaminants such as particulates and microorganisms - HEPA filtration
Entry/exit via airlock, possibly with a shower
Rounded edges of the room for easy cleaning
Continuous monitoring for contamination

Protect Products from YOU!

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Multiple Choice

_______ should be left in a locker or office before entering the lab.

• Personal belongings

• Cell phone

• Jewelry

• Food

• All of these Items

Multiple Choice

Proper laboratory attire includes

• Shorts

• Sandals

• Short sleeves

• None of these

• Loose long hair

Multiple Choice

When taking off your PPE what is the first piece of PPE that you take off?

• Googles

• Gown

• Mask

• Gloves

• Face Shield

Multiple Choice

When taking off your PPE what is the second piece of PPE that you take off?

• Googles

• Gown

• Mask

• Gloves

• Face Shield

Multiple Choice

Which of the following is a correct statement about wearing personal protective equipment such as goggles, gloves and aprons?

• wearing goggles is optional if you wear contacts or glasses.

• wearing protective equipment is optional.

• wear protective equipment when required by the activity.

• aprons are not required when wearing jeans and long sleeves.

• PPE is not needed just this one time

Multiple Select

A person has cut themselves while doing lab work. What should they do? (Choose all that apply.)

• Call 911

• Wash the cut and return to work

• Tell the lab manager

• Don't tell anyone, it's just a little cut.

• Complete an incident report

Multiple Choice

You do not need to wash your hands after working in the lab if you were wearing gloves.

• True

• False