Restriction Enzymes and DNA Analysis

Match

Match the acronyms to what they stand for.

• RFLP

• VNTR

• DNA

• PCR

Restriction Fragment Length Polymorphism

variable number tandem repeats

Deoxyribonucleic Acid

Polymerase Chain Reaction

• Restriction Fragment Length Polymorphism

• variable number tandem repeats

• Deoxyribonucleic Acid

• Polymerase Chain Reaction

Fill in the Blanks

A drawback to using restriction fragment length polymorphism (RFLP) is that you need a

sample.

Draw

A restriction enzyme uses the sequence "TCGTAG". Draw a vertical line showing where this strand of DNA would be cut in two.

Draw

A restriction enzyme uses the sequence "GGATCA" for the top strand and "AGTCGGCG" for the bottom strand. Draw a line where this cut will be made.

Multiple Choice

What is used to "cut" DNA for processing for analysis like restriction fragment length polymorphism or gel electorophoresis?

• Promoter Regions

• Restriction enzymes

• Splicing restriction polynucleases

• Nucleic polymer fragment generators

Categorize

Options (4)

Determine if the following DNA samples of blunt ends or sticky ends.

Blunt Ends

Sticky Ends

Multiple Choice

What does the polymerase chain reaction (PCR) do?

• Cuts DNA into pieces

• Separates DNA fragments by size

• Makes copies of DNA fragments for more accurate and reliable testing.

• Processes DNA fragments into proteins.

Reorder

Put the steps of PCR in order.

• Denaturing

• Annealing

• Extension

1

2

3

• 1
• 2
• 3

Multiple Choice

Why use PCR for DNA analysis instead of RFLP?

• PCR is preferred for its speed and sensitivity in amplifying DNA.
• RFLP is faster and easier to perform than PCR.
• RFLP provides more detailed information about DNA sequences than PCR.
• PCR requires more DNA than RFLP for analysis.

Multiple Choice

How many base pairs is the band for sample A?

• 1000

• 1500

• 500

• 750

Multiple Choice

How many base pairs is the first band for sample B?

• 800

• 1000

• 500

• 100

Multiple Choice

Assume the lanes are labeled 1-5 from left to right. Which two samples match?

• 1 and 5

• 2 and 5

• 2 and 4

• 3 and 4

Multiple Choice

Assume the lanes are labeled 1-5 from left to right. Which lane shows the DNA ladder?

• 1

• 2

• 3

• 4

• 5

Multiple Choice

Which suspect's DNA matches the DNA found at the crime scence?

• Suspect 1

• Suspect 2

• Suspect 3

• None of the suspects