Biotechnology Review EOC

Biotechnology Revision

Outcome 1-4 (2021)

A few questions on outcome 1

Fill in the Blanks

What is the name given to a cluster of related genes on a bacterial chromosome that are controlled by a shared promoter?

Multiple Choice

What is the name of the lac Z gene?

• Lactose Permease

• Beta galactosidase

• Transacetylase

Multiple Choice

what is the function of the Lac Y gene ( lactose permease)

• Breaks lactose into glucose and galactose.

• Helps lactose to cross the plasma membrane.

Multiple Select

Which of these are found in a typical plasmid?

• Promoter site

• Origin of Replication

• Multiple Cloning site

• Antibiotic Resistance Gene

• All of these

Multiple Choice

Which enzyme is involved in transcription?

• DNA Polymerase

• RNA Polymerase

• RNA Primase

• Helicase

Multiple Choice

What is removed during RNA splicing?

• Introns

• Exons

• Genes

• One nucleotide

Multiple Choice

Which post-translational modification involved adding carbohydrate to a protein?

• Cleavage

• Phosphorylation

• Glycosylation

A few questions on Outcome 2

Multiple Select

Which type of ends are most useful for creating recombinant DNA?

• Blunt ends

• Tacky ends

• Sticky ends

• Poly-A ends

Multiple Choice

the use of a restriction enzymes that creates sticky ends means less DNA ligase is required? true of false?

• True

• False

Multiple Choice

Select the correct order for these stages in the creation of recombinant DNA.

1 Sticky ends are complementary and pair to each other.

2 DNA ligase will join (ligate) the backbone together to form a complete double strand of DNA.

3 Two double-stranded pieces of DNA are cut with the same restriction enzyme.

4 Hydrogen bonds will form.

• 1,2,3,4,

• 3,1,4,2,

• 4,3,2,1,

• 2,4,1,3

Multiple Choice

The uptake of external genetic information by a bacterial cell, which is incorporated into their chromosome is called

• Transfection

• Transformation

• Translation

• Transcription

Multiple Choice

what is the name of the plasmid found in Agrobacterium tumefaciens, used to genetically modify a plant.

• Pi plasmid

• Fi plasmid

• Ti plasmid

A few questions on Outcome 3

Multiple Select

Which two terms could be used to describe an enclosed and sterilised vessel that maintains optimal conditions for the growth of a microorganism

• Fermenter

• Pressure cooker

• Bioreactor

• Nuclear reactor

Multiple Choice

Which of these are similarities between batch and continuous culture / fermentation processes?

• Both are large scale processes.

• Both used to produce microbial biomass or products.

• Both run under aseptically controlled conditions

• Both use large fermenter tanks

• All of these are correct.

Multiple Choice

Is batch fermentation described as...

• open system

• closed system

Multiple Choice

In batch fermentation ...

• All growth curve phases are seen.

• The process is maintained in log phase for extended periods.

Multiple Choice

Which process has the disadvantage of being more at risk from contamination due to constant adjustments?

• Batch

• Continuous

Fill in the Blanks

The separation and refinement of useful proteins into a form suitable for their end use is called '

' processing

Multiple Select

Which of these is NOT part of upstream processing

• Inoculum design

• Media choice

• Improvement of Medium

• Removal of Insolubles

• Optimisation of growth factors

Multiple Choice

What is the correct order of these processes.

1) Product polishing/finishing

2) Removal of insolubles

3) Product purification

4) Product isolation

• 1,2,3,4,

• 4,3,2,1,

• 2,4,3,1

• 1,3,4,2