Cloning Lesson

Multiple Choice

Restriction Enzymes can cut any sequence of DNA.

• True

• False

Dropdown

​

Select answer

DNA is created by using ​the ​

Select answer

from a bacteria cell and splicing a section of DNA into it.​ ​ ​ ​

Fill in the Blanks

What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate backbone?

Multiple Choice

The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....

• sticky ends

• single strands

• restriction fragments

Multiple Select

Restriction endonucleases....

• Cut DNA at a specific sites

• Can be used to isolate a gene of interest

• Protect bacterial cells by cutting foreign DNA.

Reorder

The following steps involved in gene cloning procedures. Rearrange them in descending order.

• Recombinant DNA is inserted into a cell for replication.

• Insert DNA fragment into the cut bacterial plasmid.

• Cleave the plasmid and human DNA with the same restriction enzyme.

• Isolate the bacterial plasmid and desired gene.

1

2

3

4

• 1
• 2
• 3
• 4

Multiple Select

Sticky ends are produced when the restriction enzyme cuts...

• in a staggered manner

• straight through the DNA

Match

Match the following

• Plasmid

• Genetic engineering

• Restriction enzyme

• Recombinant DNA

small circular DNA molecule

the direct manipulation of genes for practical purpose

enzyme that cuts DNA at specific recognition sites

DNA formed by combining DNA from different sources

• small circular DNA molecule

• the direct manipulation of genes for practical purpose

• enzyme that cuts DNA at specific recognition sites

• DNA formed by combining DNA from different sources

Multiple Select

DNA Cloning is used in which of the following technologies?

• Gel Electrophoresis

• Polymerase Chain Restriction

• DNA Sequencing

• DNA Mapping

Fill in the Blanks

In DNA cloning, the plasmid acts as the