RNA Processing

Multiple Choice

Which of the following is not a part of RNA processing in eukaryotic cells?

• Addition of a 5’ cap

• Removal of introns

• Addition of a poly-A tail

• Transcription of DNA to RNA

Fill in the Blank

The 5’ end of the pre-mRNA is modified by the addition of a ______, which helps protect the RNA and facilitate its export from the nucleus.

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Match

Match the following RNA processing events with their descriptions:

• Splicing

• Polyadenylation

• Capping

Removal of introns and joining of exons

 Addition of a poly-A tail to the 3’ end

Addition of a modified guanine nucleotid

• Removal of introns and joining of exons

•  Addition of a poly-A tail to the 3’ end

• Addition of a modified guanine nucleotid

Categorize

Options (5)

RNA splicing

Addition of poly-A tail

Addition of 5’ cap

Translation of mRNA

Binding of tRNA to mRNA

Categorize the following components as either part of RNA processing or not:

Part of RNA splicing

Not Part of RNA Processing

Multiple Choice

What is the role of aminoacyl-tRNA synthetase in the process of amino acid activation during translation?

• It catalyzes the formation of peptide bonds between amino acids.

• It attaches a specific amino acid to its corresponding tRNA molecule, forming aminoacyl-tRNA.

• It removes introns from the pre-mRNA.

• It adds a 5’ cap to the mRNA.

Multiple Choice

What is the primary function of the A site on the ribosome during translation?

• It holds the tRNA carrying the growing polypeptide chain.

• It binds the incoming aminoacyl-tRNA, which carries the next amino acid to be added to the polypeptide chain.

• It is the site where the tRNA exits the ribosome after releasing its amino acid.

• It is the location where the ribosome binds to the mRNA.

Fill in the Blank

The ______ site on the ribosome is responsible for holding the tRNA that carries the growing polypeptide chain during translation.

*write down full name of the site

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Multiple Choice

During the initiation stage of translation, what is the correct sequence of events that occur?

•  The large ribosomal subunit binds to mRNA, followed by the binding of the small ribosomal subunit and initiator tRNA.

• The small ribosomal subunit binds to mRNA, the initiator tRNA carrying methionine binds to the start codon, and then the large ribosomal subunit binds to form the translation initiation complex.

• The initiator tRNA binds directly to the A site of the ribosome, and the small and large subunits assemble around it.

• The large ribosomal subunit binds to mRNA first, followed by the binding of tRNA and then the small ribosomal subunit.