Mod 2.4 Enzyme Regulation

Match

Match the following Enzyme Concepts

• Enzyme

• Active Site

• Substrate

• Product

• Transition state

Protein facilitates chemical reactions

Enzyme location that binds substrate

Starting reactants bind to enzyme

End result of reaction after reaction

Energetic, unstable state in a reaction

• Protein facilitates chemical reactions

• Enzyme location that binds substrate

• Starting reactants bind to enzyme

• End result of reaction after reaction

• Energetic, unstable state in a reaction

Multiple Choice

In the lock-and-key model of enzyme action, the ___________ fits into the __________ of the enzyme.

• product; substrate

• active site; product

• substrate; active site

• activw site; substrate

Multiple Choice

Which analogy best fits for enzyme function?

• A substrate is like a skeleton key that fits into many enzymes / locks.

• An enzyme is like a teacher that does not help students learn faster or substrates transform.

• The interaction between enzyme and substrate is like a hug or handshake where the shape changes to make the interaction possible.

• Enzyme functions as the stage crew for a play of the chemical reaction where the stage crew is changed by the play.

Labelling

Label the graph showing which line represents the activation energy with or without an enzyme.

Drag labels to their correct position on the image

activation energy WITHOUT enzyme

Energy stored in reactant

Energy stored in product

Activation energy WITH enzyme

Factors that affect the microenvironment, orientation, direct participation, or strain/stretch of the enzyme-substrate complex change the enzyme activity and chemical reaction

This change might be external interaction with or internal response to the environment.

Open Ended

Brainstorm: Why do so many factors affect enzyme activity and the chemical reactions that they catalyze?

Multiple Choice

For the enzyme-catalyzed reaction shown in Figure 6.2, which of these treatments will cause the greatest increase in the rate of the reaction, if the initial reactant concentration is 1.0 micromolar?

• doubling the activation energy needed

• cooling the reaction by 10°C

• doubling the concentration of the reactants to 2.0 micromolar

• doubling the enzyme concentration

Multiple Choice

Based on the graph, what is the optimal temperature for this enzyme? 

• 15^oC
• 40^oC
• 30^oC
• 35^oC

Multiple Select

What levels of enzyme shape are affected by denaturation? Select all that apply.

• Primary

• Secondary

• Tertiary

• Quaternary

Dropdown

Allosteric means the molecule binds ​

Select answer

either increasing the rate of the reaction and lowering the activation energy as an​

Select answer

​ or decreasing the rate of reaction and increasing the activation energy as an​

Select answer

Hotspot

Identify the location where a competitive inhibitor would bind

Drag and Drop

As part of positive feedback, a cell could overcome competitive reversible inhibition by​

Placeholder

Drag these tiles and drop them in the correct blank above

increasing substrate

decreasing substrate

increasing inhibitor

decreasing enzyme

Drag and Drop

Protein folding and protein cleavage affects enzyme​

Placeholder

directly

Drag these tiles and drop them in the correct blank above

shape

location

denaturation

Dropdown

Phosphofructokinase - an enzyme that transforms glucose into energy - is inhibited by phosphorylation​

Select answer

by​

Select answer

stopping glucose metabolism as ​

Select answer

​feedback

Word Cloud

What is the evolutionary advantage of regulating enzymes? (1-2 word answer)