Microscopy Calculations - Prelab

Find the total magnification: 
Eyepiece = 10X 
Objective lens = 55X  

• 10x
• 55x
• 65x
• 550x

You should always carry the microscope by the

• arm and base
• arm and body tube
• arm only
• the objectives

When looking at a specimen on your slide, you should always start with the__________ to find your object.

• scanning objective lens
• low objective lens
• high objective lens
• oil immersion power lens

The _______is used to focus the image under scanning power.

• revolving nose-piece
• fine adjustment knob
• coarse adjustment knob
• diaphragm

Never use this on your microscope when viewing under high power.

• Fine adjustment
• Diaphragm
• Course adjustment
• Stage clips

The field of view is 1450 μm and 8 fungi fruiting bodies are observed across the diameter of your view.  What is the average size of one fungal fruiting bodies?

• 181 micrometers
• 11,600 micrometers
• .006 micrometers
• 1.81 micrometers

The field of view is 675 μm and a baby starfish ray (leg) takes up 3/4 of you view.  What is the average size of one starfish ray?

• 2,700 μm
• 169 μm
• 900 μm
• 506 μm

Here are the settings on a microscope:
Ocular Lens: 10X
Scanning  Lens: 4X
Low Power  Lens: 10X
High Power  Lens: 42X
F.O.V for 4X = 3.4 mm
Use this information to figure out big an average bacteria is if 256 fit across the field of view of the high power objective.

• 1.3 μm
• .001 μm
• 1300 μm
• 143 μm

Tags

NGSS.MS-LS1-1

Here are the settings on a microscope:
Ocular Lens: 10X
Scanning  Lens: 4X
Low Power  Lens: 15X
High Power  Lens: 50X
F.O.V for 4X = 3.4 mm
You are viewing human blood under low power.  How much bigger do those cells appear to you through the eyepiece. (compared to what they are in real life)

• 500x
• 10x
• 150x
• 15x

Here are the settings on a microscope:
Ocular Lens: 10X
Scanning  Lens: 4X
Low Power  Lens: 10X
High Power  Lens: 40X
F.O.V for 4X = 3.4 mm
You are viewing human blood under high power. You need to calculate the average size of a red blood cell.  You estimate that 51 cells will fit across your F.O.V.  What is the average size of one of those red blood cells?

• about 60 μm
• About 6 μm
• about 2040 μm
• about 1μm