Biotechnology Review

Considering the ethical issues surrounding the use of DNA technology,what is the main goal of the Human Genome Project?

• to discover a new medicine for cancer
• to understand how the human body heals
• to determine the genetic makeup of humans
• to determine how the human digestive system functions

During gel electrophoresis, where would the shortest pieces of DNA be found?

• The top of the gel
• The bottom of the gel
• The middle of the gel
• Scattered throughout

Which would include the use of DNA to determine paternity?

• test cross
• gene therapy
• recombinant DNA
• DNA fingerprinting

How can gene therapy positively impact society?

• by producing insulin using bacteria
• by creating fingerprints of criminals
• by mapping all genes in the human population
• by treating genetic disorders with the insertion of normal genes

What do scientists hope to accomplish through stem cell research?

• cloning humans
• developing vaccines for lead poisoning
• growing organs for transplant surgery
• developing restriction enzymes to cut DNA

Which best describes a transgenic organism?

• clones produced from the body cells of an organism
• organisms that provide tissues and organs for transplants
• DNA and RNA artificially produced in the cells of an organism toprotect the immune system
• genes from other organisms inserted into a different organism toincrease the genetic variability

Which steps are in the correct order to create insulin-producing bacteria?

• cut bacterial plasmid with restriction enzyme, insert human insulinproducinggene, seal with ligase enzyme
• seal with ligase enzyme, cut bacterial plasmid with restrictionenzyme, insert human insulin-producing gene
• cut bacterial plasmid with restriction enzyme, seal with ligaseenzyme, insert human insulin-producing gene
• insert human insulin-producing gene, cut bacterial plasmid withrestriction enzyme, seal with ligase enzyme

Which genes would most likely be inserted into a crop to aid in its survival against insects and low temperatures?

• freeze resistance and increased yield
• freeze resistance and pest resistance
• heat resistance and increased yield
• heat resistance and pest resistance

Which term applies to an organism that contains more than one source ofDNA?

• mutation
• translocation
• transgenic organism
• polymorphic organism

Which best describes recombinant DNA?

• DNA that has been deactivated by enzymes
• DNA found in organelles other than the nucleus
• inserted genes from one organism into another to direct it to produced protein
• genes developed when an egg nucleus and a sperm nucleus fuse to form a zygote