Genetics Review-a-thon

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

What applications can gel electrophoresis be used for?

• Parental Testing
• Criminal Investigations
• Sequence Endangered Species DNA
• All of the above

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

IN PCR why is the DNA heated?

• To split the DNA strand

• To remove the DNA strand

• To correct the DNA strand

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.
• Cambodia
• The rain forest
• The CDC

What happens in the Denature step of PCR?

• The DNA nucleotides are broken apart.
• The base-pairing rules for DNA are reversed.
• The double-stranded DNA is separated into two single strands of DNA.
• The DNA is returned to its natural setting.

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

What is the purpose of the Extension step of PCR?

• To create the primers.
• To extend the time it takes to produce DNA.
• To allow polymerase to create the complementary strands of DNA.
• To allow substrates to copy DNA sequences.