Chapter 9 Biotechnology Review

In the gel below which DNA fragment is the largest?

Hint: Pay attention to the direction of travel

• A

• B

• C

• D

In the gel below what process is used to separate fragments?

• ultraviolet radiation

• electricity

• restriction enzymes

• PCR

What is a polymerase chain reaction use for?

• to make many copies of DNA

• to separate DNA based on size

• to cut DNA into fragments

• to make restriction enzymes

What does DNA fingerprinting compare?

• each gene making up a person's DNA

• the proteins DNA codes for

• repeating units in coding segments of DNA

• repeating units in noncoding segments of DNA

What four things are needed in a PCR?

• DNA to be copied, DNA polymerase, nucleotides, helicase

• DNA to be copied, DNA polymerase, nucleotides, 2 primers

• DNA to be copied, RNA polymerase, nucleotides, ligase

• DNa to be copied, RNA polymerase, 2 primers, helicase

Why is heat used in a PCR?

• to speed the process up

• reattaches the DNA double helix

• separates the original DNA strand so it can be copied

• adds free floating nucleotides to the new DNA strand

How much DNA is made each PCR cycle?

• doubles

• quadruples

• splits in half

• maintains the same amount

How long are the DNA strands that result from being treated with restriction enzymes?

• 2-3 base pairs

• 4-8 base pairs

• 10-20 base pairs

• unlimited base pairs

Which of the following is a potential adverse (negative) effect of genetic engineering?

• Produce medicines

• Save engaged species

• Higher crop yield

• Decreased genetic diversity

What is B?

• Plasmid

• Foreign gene