HPLC CLB 10803

What does chromatography means?

• A technique of separation of mixture to their elements

• A study of interaction between light and matter

• An equipment that only capable in providing quantitative analysis

Choose the right statement that best differentiates between HPLC and GC.

• Both have a column that served as the stationary phase

• HPLC separates mixture based on their polarity while GC separates based on the solubility of the mixture

• Both have normal and reverse phase operation

What does 'normal phase' means?

• Polarity of the mobile phase is higher than stationary phase

• Both stationary and mobile phase have the same polarity

• Polarity of the stationary phase is higher than mobile phase

Which one of these do not associate with the HPLC component?

• UV detector

• Pump

• Degasser

• Atomizer

Choose the wrong characteristics of the eluent that can be used in the HPLC

• Must be pure

• Can be used without degassing

• Must complied to HPLC standard

• May have constant or gradient polarity

Choose two methods commonly used in degassing gas

• Helium purge method

• Gas-gas liquid separation membrane method

• Gas-liquid separation membrane method

Choose the wrong statement regarding the column.

• Usually made of stainless steel material

• Packing materials commonly used are silica or resin

• Longer column will give longer time for the mixture to separate and thus gives better resolution

• Higher column ID can provide higher separation sensitivity

Which one of these that is not the common detector in HPLC?

• Infra Red

• Ultraviolet light

• Flourescent

• Mass Spectroscopy

What can be done to optimize the separation?

• Adjusting operational variables

• Careful selection of the column format

• Calibration of the HPLC before conducting any analysis

• Selecting the optimum temperature for the separation

Who is the Father of Independence of Malaysia?

• Tunku Abdul Rahman Putra Al Haj

• Tun Haji Abdul Razak bin Dato' Haji Hussein

• Tun Haji Hussein bin Dato' Haji Sir Onn

• Tun Haji Abdul Ghaffar bin Baba