Biosafety

Below is the list of laboratory rules except

• Wear lab coat, glove and cover shoes every time entering into lab

• Never do any experiment without instruction by laboratory instructor/technician

• Eating, drinking and smoking are prohibited inside the laboratory

• Student can made noise during discussion inside the laboratory

You are asked to handle the concentrated H2SO4 by your instructor. What precaution should you do while handling it?

• Pour the acid inside the fume hood, wearing the protective glove and lab coat.

• Running the experiment using that acid on the bench.

• Pour the acid without wearing glove inside the fume hood

• Add the acid with 100% H2O2 inside the fume hood.

During the experiment with bioreactor, you are required to take the sample using the syringe. After finish sampling, what should you do with that syringe?

• Wash it and let it dry on the table.

• Syringe is consider as single use consumable, it should be dispose inside the biohazard bin after used.

• Wash and keep it as souvenir.

• Just left it on the table.

What is the proper way to handle acid?

• Add acid to water.

• Add H2O2 into 2M of HCl

• Pour water into acid

• Add 2M of H2SO4 into H2O2

When running the experiment, you are planning to continue doing your analysis on the next day. You have taken the sample and want to store it in the chiller. How you should label your sample?

• Just label it with "sample A' and stored it

• Put your name and date.

• Write your name and matric no., sample name, date of collected, estimated expired date, class/course, instructor name.

• Just put your group no and date.

All of these are the step you will do in the time of chemical or bacterial sample spill on the table except

• Use the spill kits to stop the spill from spreading to other area.

• Clean using dry towel and spray it with 70% ethanol for disinfectant.

• Let the spill spread and drop into the floor and flooding the area

• Alert the lab instructor/technician about the spill.

Which one is the signage for "Extremely Flammable" that you can found in some chemical?

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What is the hazard that you may found in the lab.

• Chemicals

• Infectious bacteria

• physical hazard such as falling from the wet floor

• All the listed above

What is the classification of Biosafety level 1 laboratory

• Involve with no infectious bacteria

• The lab activities consider low risk to community

• Involve any infectious disease bacteria but will not harm to community.

• Involve any infectious disease bacteria and easily been spreading due to contact from one individual to another.

What is Containment Level 1

• Need to have special fume hood with special ventilation to avoid spreading the disease to outside environment.

• Required special and well-design facilities with HEPA filter and strict laboratory access.

• This level of containment represents an isolated unit, functionally and, when necessary, structurally independent of other areas.

• Required no special design lab and work can be done on the bench. Example is basic microbiology lab.