Recombinant DNA Technology

Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the tools below is used to cut the gene from its normal location?

• Bacteriophage

• Vector

• Restriction enzyme

• Plasmid

Palindromic sequences in DNA

• Reflect the same sequence on two opposite sides

• Are not useful in recombinant DNA experiments.

• Form "blunt" ends when cut by restriction enzymes

• All of the above.

Restriction enzyme cleave DNA by cutting

• The covalent bond in the nitrogenous base

• The hydrogen bond between the nitrogenous base pair

• The glycosidic bond between the deoxyribose sugar and nitrogenous base

• The phosphodiester bond between the deoxyribose sugar and phosphate group

Which of the following is a protein that acts as DNA scissors?

• plasmid
• restriction enzyme
• ligase
• protein expression

Which of the following is an enzymatic protein that acts as DNA glue?

• plasmid
• restriction enzyme
• ligase
• protein expression

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.
• Plasmids contain a promoter sequence that defines where transcription begins.
• Plasmids can contain an antibiotic resistance gene.
• All of the statements are true about plasmids.

In bacteria, restriction enzymes are used for defense. They cut up the DNA of a virus that infects bacteria.

• True
• False

Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.

• polymerase
• ligase
• dehydrogenase
• lactase

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• rDNA technology
• transferrence
• transformation
• rDNA plasmid

Which of the following model organisms is often used in rDNA technology to make insulin?

• small squid
• mouse
• fruit fly
• E. coli