Biology Honors-CHAPTER 12 (Modules 12.1-12.5: Gene Tech)

Biotechnology

• Manipulation of genes for practical purposes

• Manipulation of organisms or their components to make useful products

• A gene of interest which is copied using DNA technology

• Using modering technology to help benefit the environment

How is gene cloning possible?

• Scientists are using modern laboratory techniques used to studying and manipulating genetic material (DNA Technology)

• Manipulating the mitochondria in order to produce ATP faster so the cell can divide rapidly

• Manipulating the enzymes that produces polypeptides

Plasmids...

• Small circular DNA found in eukaryotic cells

• Have bacterial enzymes that cut at specific sequences

• Can be transferred into bacterial cells

• Small circular DNA found in bacterial cells

Which of the following statements about Recombinant DNA is false?

• Formed when 2 different sources of DNA is combined

• Recombinant DNA technology is used today for genetic engineering

• Recombinant DNA re-joined together by the same strand

What is the FIRST 3 STEPS of gene cloning?

• Transformation occurs, gene of interest is isolated, gene V is activated

• DNA ligase is added to join plasmid and gene of interest after plasmid and gene of interest is isolated

• Gene of interest is cut, protein is deactivated, RNA ligase is activated

• Plasmid is isolated from Vector, gene of interest is isolated, plasmid is cut using a restriction enzyme

What are the NEXT 3 STEPS of gene cloning AFTER STEPS 1-3?

• Gene of interest is cut using the restriction enzyme, DNA ligase is added to join plasmid and gene of interest, cut plasmid and cut gene of interest are mixed

• Recombinant bacterium replicates to form a clone of cells and gene cloning can be used for immediate product and protein from gene

• Gene of interest is cut using the restriction enzyme, cut plasmid and cut gene of interest are mixed, DNA ligase is added to join plasmid and gene of interest

• DNA ligase is added to join plasmid and gene of interest, Gene of interest is cut using the restriction enzyme, cut plasmid and cut gene of interest are mixed

What are the FINAL STEPS of gene cloning?

• Recombinant plasmid and bacteria are mixed and plasmid undergoes transformation, recombinant bacteria replicates to form a clone of cells which can be used for immediate product or for a protein

• Recombinant bacteria replicates to form a clone of cells which can be used for immediate product or for a protein, recombinant plasmid and bacteria are mixed and plasmid undergoes transformation

Restriction enzymes...

• Bacterial enzymes that cut DNA at specific sequences

• that biologists discovered have hundreds of different restriction enzymes

• Are basically molecular scissors

• All following choices are true

A restriction site is also known as a _____________.

• cut site

• repairing site

• nucleus

• None of the above

Which of the following statements of restriction sites is true?

• It has a 4-8 nucleotide pair sequence that restriction enzyme recognizes and is unique to each restriction enzyme.

• It cuts parts of the RNA randomly to be spliced into new coding sections

• It has a 4-8 nucleotide pair sequence that restriction enzyme recognizes and is the same to each restriction enzyme.

• It cuts parts of the DNA randomly to be spliced into new coding sections