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Chapter 6 HPLC

Authored by Nurul aili Zakaria

Biology

University

Used 243+ times

Chapter 6 HPLC
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14 questions

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1.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

HPLC methods include

liquid/liquid (partition) chromatography

liquid/solid (adsorption) chromatography

ion exchange nd size exclusion chromatography

all of the option correct

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The substance in the mixture that has the greatest affinity for the mobile phase will travel

fastest
slowest

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following can be used to find the concentration of a substance in HPLC

Rf value
Rt value
peak area
peak width

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What two properties affect the Rt of a compound?
I Size/weight
II Polarity
III melting point

I only
II only
III only
I and II

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

In order to compare to chromatograms what must be kept the same?

stationary  phase
mobile phase
temperature
all of the above

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Based on a normal chromatography, the stationary phase is polar.

TRUE

FALSE

Depends on the substance

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What does 'normal phase' means?

Polarity of the mobile phase is higher than stationary phase

Both stationary and mobile phase have the same polarity

Polarity of the stationary phase is higher than mobile phase

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