HEMAGY2: Reticulocyte Count

Which of the following samples may be utilized for reticulocyte count? (more than one answer required)

• EDTA blood

• citrated blood

• capillary blood

• oxalate anticoagulated blood

• heparinized blood

Which of the following stains are able to demonstrate reticulocyte granules? (more than one answer required)

• New methylene blue

• Wright's stain

• Prussin blue

• Brilliant cresyl blue

Presence of RBC inclusions results in falsely elevated reticulocyte counts.

• True

• False

• Maybe

• I don't know

An immature erythrocyte in peripheral blood must have at least 4 deep blue granules to be considered as a reticulocyte.

• True

• False

• Maybe

• I don't know

Adorable Mhadoxx performed a reticulocyte count on the sample of a 1-month-old infant . He utilized the Calibrated Miller Disk method and counted 24 reticulocytes out of 500 erythrocytes. Which of the following is CORRECT regarding the reticulocyte count of the patient? (more than one answer required)

• Retic count is 0.5%

• Retic count is 2.5%

• Retic count is decreased

• Retic count is normal

How many erythrocytes are counted in the routine light microscope method of reticulocyte count?

• 1000

• 500

• 100

• 50

Which of the following statements about reticulocytes are TRUE? (more than one answer required)

• Contains ribosomal RNA observable using a supravital stain.

• Stays in the marrow for 2-3 days prior to release into blood.

• Seen as polychromatophilic erythrocyte in Wright's stain.

• Ribosomal RNA appears as blue-stained structures in Wright;s stain.

In the calibrated Miller disk method of reticulocyte count, cells that appear as light to medium green granules in the smaller square are counted as

• erythrocytes

• reticulocytes

• both

• neither

What erroneous result is observed in the reticulocyte count of a patient suffering from uncontrolled diabetes mellitus?

• falsely increased

• falsely decreased

• variable

• no effect

Feverish Felix performed a reticulocyte count on a sample even if he is not feeling well. After incubating the stain-sample mixture for 10-15 minutes, he immediately prepared smears from the bottom portion of the mixture. Which of the following is the MOST LIKELY erroneous result?

• falsely increased

• falsely decreased

• variable

• no effect