Bio Unit 4 Topic 1 - F

What is recombinant DNA?

• Adding DNA from one organism into the DNA of another
• DNA which has been changed over generations by natural selection
• DNA that causes genetic disorders
• DNA that has been sequenced

Tags

NGSS.HS-LS3-2

What is a restriction enzyme?

• Enzyme that cuts DNA
• Enzyme that add to the DNA strand
• Enzyme that builds proteins
• Enzyme that breaks down lipids

How do you create recombinant DNA?

• First, cut the gene and plasmid with the same restriction enzyme. Second, insert the gene into the plasmid. 
• First, insert the gene into the plasmid.  Second, cut the gene and plasmid with the same restriction enzyme. 
• First, cut the gene and plasmid with different restriction enzymes. Second, insert the gene into the plasmid. 
• First, insert the gene into the plasmid.  Second, cut the gene and plasmid with different restriction enzymes. 

Creating a transgenic organism is an example of _____________. 

• PCR
• Genetic Engineering
• Gel Electrophorsis
• Gene Therapy

Which is a human protein produced by bacteria as a treatment for diabetes?

• insulin

• Diatase

• Collagen

• Hemoglobin

Who is the father?

• Dad 1
• Dad 2
• Dad 3 
• can not be determined

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

How do you create recombinant DNA?

• First, cut the gene and plasmid with the same restriction enzyme. Second, insert the gene into the plasmid. 
• First, insert the gene into the plasmid.  Second, cut the gene and plasmid with the same restriction enzyme. 
• First, cut the gene and plasmid with different restriction enzymes. Second, insert the gene into the plasmid. 
• First, insert the gene into the plasmid.  Second, cut the gene and plasmid with different restriction enzymes. 

Why are primers necessary for PCR?

• They help separate the double helix
• They are a starting point for DNA polymerase
• They help anneal the newly formed strands
• They bring in new nucleotides

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

By looking at a restriction map, we can tell the size, but NOT the DNA sequences themselves.

• True
• False