primer designing of pcr

Primers containing highly repetitive sequence are prone to generate ------ when amplifying genomic DNA

• non-specific amplicons

• specific amplicons

• repetitive amplicons

• all of there

To improve specificity of the primers it is necessary to avoid -----

• regions of cross homology

• regions of homology

• self dimers

• hetrodimers

To improve specificity of the primers it is necessary to avoid regions of homology

Commonly, primers are designed and then ---- to test the specificity

• genscaned

• tested

• BLASTed

• all of these

Parameters for primer pair design-----------------

For Real Time-PCR, the target length is closer

• 100-200 bp

• 50-60bp

• 30-70bp

• 200-300bp

For standard PCR, it is near

• 300-400bp

• 1-50bp

• 500-1000 bp

• 30-70bp