Nucleic acid Probes

What is a probe?

• Chemically synthesized DNA

• Purified DNA

• Fragmented DNA duplex

• Either purified or synthesized single stranded DNA

Why is a probe labeled?

• Improve visibility

• Improve stability

• Improve location identification

• Improve binding capability

Nucleic acid hybridization is used to identify _________

• RNAs

• DNAs

• Complementary base sequences

• Proteins

The procedure by which DNA gel is transferred to a membrane is termed as ___________________

• blotting

• centifugation

• electrophoresis

• paper chromatography

Klenow fragment is derived from

• DNA Ligase

• DNA Pol-I

• DNA Pol-II

• Reverse Transcriptase

Which of the following does not affect the formation of hybrid DNA?

• Ionic strength

• Pressure

• Temperature

• Homologous DNA

Which of the following is not termed as hybridization?

• DNA and cDNA

• DNA and mRNA

• DNA from different species

• DNA from male and female of same species

cDNA Probes can be obtained by reverse transcription of mRNA

• True

• False

Which of the following probe is generally used for screening cDNA libraries

• DNA probe

• RNA probe

• cDNA

• Oligonucleotide probes

Long probes are usually made of ---------

• Gene expression

• Cloning

• Hybridization

• PCR