Recombinant DNA technology

what are the tools used in rDNA technology

• Restriction enzymes

• DNA ligase

• vectors

• DNA helicase

Transposons are also known as

• jumping genes

• Expression vector

• Molecular scissor

• Biolistic gun

pUC 19 is a

Choose the odd one out

• microinjection

• liposome mediated gene transfer

• biolistic gun

• Ti -Plasmid vecotr

the reporter gene that is responsible for blue white colony in transformation is

pBR 322 has two antibiotic resistance genes namely a) tetracyline and b) ampicillin

• only a is correct

• only b is correct

• both a and b correct

• both and b wrong

cosmid vector is a

• vector with cos sites of lambda phage and DNA from plasmid

• has only cos sites of plasmid DNA

• has only cos sites of lambda phage

• all are incorrect

Which of the following is an enzymatic protein that acts as DNA glue?

• plasmid
• restriction enzyme
• ligase
• protein expression

The unpaired nucleotides produced by the action of restriction enzymes are referred as

• sticky end

• base sequence

• single strands

• restriction fragments

Why are plasmids sometimes given an antibiotic resistance gene?

• So that scientists can make antibiotic resistant bacteria and make more money for pharmaceutical companies.
• So that scientists can create super bugs.
• So that scientists can identify which cells have the rDNA plasmid.
• So that scientists can kill the bacteria.