DNA manipulation tools

This cuts DNA at specific sequences

• Plasmid
• Recombinant DNA
• Restriction enzyme
• Gel electrophoresis

This diagram represents which of the following? 

• Gel electrophoresis
• Gene therapy
• Cloning 
• Genetic engineering

The manipulation of DNA is called

• Genetic engineering
• Karyotype
• Plasmid
• Gel electrophoresis

How do restriction enzymes "know" where to cut DNA

• A specific sequence of nucleotides

• They cut randomly

• Distance from the centromere

"Sticky-ends" are DNA strands with:

• single-stranded ends

• ends cut flat by an enzyme

• no way to be recombined

Which is used to cut DNA molecules at specific points?

• gel electrophoresis
• PCR analysis
• a restriction enzyme
• recombinant DNA

Which 2 molecules form the sides (backbone) of the DNA ladder?

• deoxyribose sugar and adenine
• deoxyribose sugar and a hydrogen bond
• deoxyribose sugar and the nucleus
• deoxyribose sugar and phosphate

DNA codes for...

• bases
• proteins
• amino acids
• phosphates

Tags

NGSS.HS-LS1-1

NGSS.HS-LS1-6

Enzyme that connects the DNA to the backbone (glues)

• Ligase

• Polymerase

• Helicase

• Primase

DNA polymerase moves in the _________ to ____________ direction

• 3' to 5'

• easiest

• 5" to 3"

• 9 to 5