western blotting

A student researcher overexpresses an exogenous protein in cell culture and wants to determine if that protein, is in fact, overexpressed. What technique would best demonstrate that this protein is expressed in these cells?

• Northern blot

• Southern blot

• Western blot

• Eastern blot

After proteins are run on an SDS-PAGE gel, a transfer is executed. What is the purpose of the transfer in Western blot protocol?

• Denature the proteins in the sample

• Visualize the proteins run on the gel

• Move proteins from the SDS-PAGE gel to a nitrocellulose membrane

• Probe the gel with an antibody to detect a protein of interest

Which of the following probes are most commonly used in Western blotting?

• Nucleic acids

• Bovine serum albumin

• Antibodies

• Nonfat dry milk

What is the purpose of "blocking" the nitrocellulose membrane during western blotting?

• To encourage non-specific binding

• To prevent non-specific binding

• To improve binding of secondary antibody

• Scientists like extra work

Southern blot used for

• RNA analysis

• DNA analysis

• Protein analysis

• Amino acid analysis

True or false: In western blotting proteins are detected through autoradiography methods with non-fluorescent compounds

• True

• False

Southern blot is the technique for _____________ blot

• RNA

• Lipid

• DNA

• Protein

In Southern blotting, ___________ type paper is used for blot transfer

• Nitrocellulose membrane or Nylon membrane filter

• Whatman filter paper

• Paper towel filter paper

• None

_________ in 1975 developed the procedure for DNA transfer or for the DNA blot

• Kary Mullis

• E. M. Southern

• Alac Jeffry

• Georges & Cesar

A scientist is using a blotting technique to measure expression of RNA amongst genes. What blotting technique is he using?

• Southern blotting

• Western blotting

• Eastern blotting

• Northern blotting