Cell and Tissue Microscopy (AS Biology)

A microscope has a resolution of 200nm. Which of the following organisms would not be resolved using this microscope?

• Chloroplast
• lysosomes
• mitochondria
• ribosomes

Which group of structures are visible in a suitably stained plant cell using a high power (x400) light microscope.

• A

• B

• C

• D

Which size ranges can be viewed using a light microscope?

• 4 only

• 1 and 2 only

• 2 and 3 only

• 3 and 4 only

The diagram shows a stage micrometer, with divisions 0.1mm apart, viewed through an eyepiece containing a graticule. What is the area of the field of view of the mciroscope at this magnification (π=3.14)

• π=12.5 x 12.5 = 4.9 x 10²μm²

• π=50 x 50 = 7.9 x 10³μm²

• π=125 x 125 = 4.9 x 10⁴μm²

• π=250 x 250 = 2.0 x 10⁵μm²

An electron microscope has a higher resolution than a light microscope. Which is a result of the higher resolution?

• the ability to produce larger images of cells
• the ability to see cristae in mitochondria
• the ability to see mRNA in all cells
• the ability to see the nucleus in eukaryotes

Eyepiece graticules and stage micrometers are used to measure cells. Which is the correct reason why an eyepiece graticule is calibrated?

• An eyepiece graticule can be used to make measurement
• An eyepeice graticule is magnified by the objective lens
• An eyepiece graticule magnifies the specimen
• An eyepiece graticule makes comparison

What best describes an electron microscope in comparison to a light micrsocope?

• higher magnification and higher resolution
• higher magnification but lower resolution
• lower magnification but higher resolution
• lower magnification and lower resolution

At approximately which magnification is light microscopy not suitable because the resolution becomes too low?

• x100
• x200
• x400
• x1500

The diagram shows a stage micrometer viewed with an eyepiece graticule scale, using a magnification of x400. Using the same magnification, a chloroplast is measured and found to be 4 eyepiece graticule divisions long. How long is the chloroplast?

• 1.0 x 10¹ μm

• 4.0 x 10² μm

• 2.5 x 10^-1 μm

• 2.5 x 10^-2μm

The eyepiece lens of a microscope is fitted with an eyepiece graticule. Which statement about the graticule are correct? (1) it allows you to measure the actual length of cells (2) it allows you to draw cells with correct proportions (3) it changes in size as the objective lens changes from x10 to x40

• 1, 2 and 3
• 1 and 3 only
• 1 only
• 2 only