DNA Extraction Methods Quiz

What is the main principle of the Phenol-chloroform method?

• DNA is separated based on density.

• DNA is separated based on charge.

• DNA is separated based on differential solubility.

• DNA is separated based on size.

Which reagent is NOT used in the Silica Gel Method?

• Lysis buffer

• Silica gel columns

• Ethanol

• Chloroform

What is the role of Proteinase K in the Proteinase K method?

• To lyse cells.

• To break down proteins.

• To precipitate DNA.

• To bind DNA to silica.

In the Salting out method, what is used to remove proteins?

• Chloroform

• Sodium chloride

• Ethanol

• Phenol

What is the purpose of magnetic beads in the Magnetic Beads method?

• To precipitate DNA.

• To bind DNA and wash away contaminants.

• To lyse cells.

• To stabilize DNA.

Which method is commonly used for forensic samples?

• Paper method

• Phenol-chloroform method

• Proteinase K method

• Silica Gel Method

What is the main application of the Paper method?

• High-throughput sequencing

• PCR and cloning

• Clinical diagnostics

• Field research and DNA archiving

What is the first step in the Phenol-chloroform method?

• Lysis of cells

• Centrifugation

• Binding of DNA to silica

• Precipitation of DNA

Which of the following is a key component of the Silica Gel Method?

• Sodium chloride

• Ethanol

• FTA paper

• Magnetic beads

What is the final step in the Proteinase K method?

• DNA is precipitated with sodium chloride.

• DNA is separated using a magnet.

• DNA is embedded in paper fibers.

• DNA is washed with ethanol.