Unit-V Animal Cell Culture

What is a cell line?

• Transformed cells

• Multilayer culture

• Sub culturing of primary culture

• Multiple growth of cells

Disaggregating of cells can be achieved by

• Enzymatic digestion

• Physical disruption

• Treating with chelating agents

• All of the above

The human fibroplast is a classical example of

• Stable primary cell lines

• Established cell lines

• Cell transformations

• None of these

Subculturing a cell line always increase the passage number. Is this true or false?

• True

• False

A cell line always requires enzymatic dissociation before it can be distributed between new culture vessels.

• True

• False

Senescence refers to the process by which a cell line is found to contain chromosomes characteristics of another cell line due to cross-contamination

• True

• False

Cross-contamination is

• Not to much of a problem and never happens

• The result of working with multiple cells at one time

• Can be controlled with antibiotics

• Cannot really happen if you follow good protocols

Trypsin is used for dissociating the tissue into single cells.

• True

• False

In the secondary culture, cells are obtained from _______________________

• Primary culture

• Organ culture

• Phenotypic culture

• None of the above

Which of the following statements describes suspension cells?

• Suspension cells do not need to be dyed by trypan blue.

• Suspension cells clump together.

• Suspension cells do not stick to a solid substrate.

• None of the above