CHEM 416: Flow Cytometry

What are the 2 types of scatter

• Forward and Backward

• Forward and Side

• Backward and Side

• Backward and Diagonal

What is side scatter proportional to?

• size only

• internal complexity only

• size and internal complexity of the cell

• None of the above

What is forward scatter proportional to?

• size only

• size and internal complexity of the cell

• internal complexity only

• none of the above

What is the order that the sample goes through?

• Fluidics, optics and filters, lasers, detector, computer system

• Fluidics, detector, lasers, optics and filters, computer system

• Lasers, optics and filters, fluidics, detector, computer system

• Fluidics, laser, optics and filters, detector, computer system

How many particles per second are modern flow flow cytometers able to analyze?

• 100 particles per second

• 500 particles per second

• 1000 particles per second

• 10000 particles per second

Flow cytometer is similar to microscope but..

• It produces high throughput, automated quantification is specified optical parameter on a cell by cell basis

• Produces an image of the cell

• Analyses the cell though a low throughput, and a used the cells all at once

• Can’t produce an image at all

The flow cytometry is able to precisely measure the quantities of antigens and the possibility to stain each cell with multiple antibodies-fluorophores.

• True

• False

What method does flow cytometry use to detect and measure cells?

• FACS- fluorescence activated cell sorting

• Electrical resistance

• Coulter cell

What is the method of analysis within a flow cytometer?

• Laser is used to detect changes in the spin of within a heterogenous mixture

• The cells flow in a narrow stream in front of laser beam, once laser beam hits them; each singular cell will be detected by light detectors when the light is reflected from them

• Laser continuous wave output disrupts cells and the energy difference is measured

Flow cytometry is a multiparametric analysis, which allows for simultaneous analysis of thousands of particles per second.

• True

• False