BMS34010A_Proteins quantification
Authored by Tania Tahtouh
Biology
University
Used 36+ times
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8 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
At _____nm wave length, we can roughly measure the concentration of protein.
220
230
260/280
280
260/230
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Biuret assay is based on ____ interaction with protein
Cu2+
Brillant blue
Folin-Ciocalteu reagent
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Quantification of protein using a UV spectrophotometer is based on ____
UV light is absorbed by the backbone of the protein
UV light is absorbed by the peptide bonds of the protein
UV light is absorbed by the aromatic amino acids of the protein
UV light is absorbed by all the non-aromatic amino acids of the protein
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The primary component in Biuret assay detects ______ of the proteins
Amino acids
Peptide bonds
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Bradford Assay is based on ____ interaction with protein
Cu2+
Coomassie Blue
Folin-Ciocalteu reagent
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
A particular enzyme assay measures____
The depletion of substrate and the formation of the product at the same time
The depletion of substrate or the formation of the product
The formation of intermediate of the reactions
The concentration of the enzyme
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
The concentration of an 'unknown protein' is usually compare with a standard curve with Y-axis is OD, and X-axis is the protein concentration
True
False
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