Rec DNA Tech

What is the purpose of amplification?

• to screen for recombinant DNA

• to join the rDNA with the host cell

• to produced multiple copies of the desired gene

• to prevent the bacterial from reproducing

Which of the following is correct about host cell?

• plasmid is an example of host cell

• un able to receive DNA molecule through transformation

• an organism that prevents cloning vectors from multiplying

• an organism that is used to receive recombinant DNA for cloning purposes

Restriction enzymes that found in bacterial cells are ordinarily used;

• during DNA replication

• to degrade the bacterial cell's DNA

• to degrade viral DNA that enters the cell

• to attach pieces of DNA together

The correct sequence of steps in gene cloning is,

• isolation, cutting, insertion, transformation and amplification, screening

• isolation, insertion, cutting, transformation and amplification, screening

• isolation, cutting, insertion, transformation, screening

• isolation, cutting, insertion, amplification, screening

In which step, DNA ligase is used?

• cutting

• insertion

• transformation

• isolation

In which step, recombinant plasmid is formed?

• cutting

• insertion

• transformation

• isolation

Where does plasmid is isolated?

• from bacterial cell

• from human cell

• from gene of interest

• from virus

What is the function of plasmid?

• as vector

• as host cell

• as target DNA

• as gene of interest

What is the function of bacteria?

• as vector

• as host cell

• as target DNA

• as gene of interest

The second step in gene cloning is,

• insertion

• cleavage of DNA

• production of recombinant DNA

• cloning