PCR 1

Specific to the forward and reverse "ends" of the piece of DNA that will be amplified. These are two small pieces of DNA between 10-30 base pairs (bp) long.

• primers

• DNA polymerase

• Taq polymerase

• nucleotides

• buffer

The enzyme that copies DNA in a PCR reaction. This enzyme is from thermophillic bacteria, originally discovered in Yellowstone.

• primers

• DNA polymerase

• Taq polymerase

• nucleotides

• buffer

Added to the growing chain of DNA, dATP, dCTP, dGTP, and dTTP (sometimes abbreviated as A, C, G, T).

• primers

• DNA polymerase

• Taq polymerase

• nucleotides

• buffer

These are steps in the PCR reaction- check all that apply

• Binding

• Annealing

• Sequencing

• Denaturation

• Extension

This is the first step of a PCR cycle which separates the DNA strands and allows the primers to bind.

This is the second step of the PCR cycle when primers bind to the DNA strand.

This is the third step in the PCR cycle when the polymerase adds nucleotides to the primer that are complimentary to the sequence.

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