Engineer it lab quiz

What is the difference between cells and bacteria in the context of this experiment?

• cells are not engineered, bacteria are

• Nothing, the words are used to mean the same thing in the instructions

• bacteria are not engineered, cells are

Before you begin you should

• label your plates

• put the antibiotic capsule in the bottle of water

• heat the water

N.S. stands for *

• non selective

• no standard

• natural selection

• no stock

your selective plates should contain what?

What temperature does heat shock happen at?

• 42 C=107F

• 38C=98.6F

• 4C=32 F

In the context of this experiment a 'plate' is

• agar

• Petri dish

• bacteria

What are the key pitfalls to avoid?

• Not seeing DNA in the loop before adding it to the cells

• Incubating blanks cells at 37°C for more than 24 hours before making them chemically competent

• Not incubating the engineered cells at 42°C

• Not boiling the water until you see bubbles when making the agar plates

• Not mixing the Blank cells to the Transformation buffer while the tube is on 'ice'

What should be done first?

• Make cells chemically competent

• make agar plates

• add DNA Plasmid

After you make the agar plates you should

• make the cells competent

• streak blank cells

• add DNA

After you streak the blank cells you should

• Make cells chemically competent

• Recover engineered cells