PLTW Medical Interventions 2.1

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.
• Cambodia
• The rain forest
• The CDC

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?

• 1
• 10
• 30
• Over 1 billion

2.1.1 Two abnormal alleles must be inherited for an individual to show symptoms of this type of genetic disorder.

• Single gene recessive

• Single gene dominant

• Single gene sex-linked

• mitochondrial

2.1.1 Type of genetic testing that would be most appropriate for parents planning to have children and have a recessive disorder in their family. The parents would like to know the probability that they may pass the disorder on to their future child.

• Diagnostic testing

• Prenatal testing

• Diagnostic testing

• Carrier testing (genotyping)

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

2.1.2 Which of the following is NOT an "ingredient" included in the process of PCR.

• Helicase enzyme

• Free nucleotides

• Primers

• taq polymerase enzymes

2.1.2 During which phase of PCR is taq polymerase active?

• Denaturation

• Annealing

• Extension

• Cloning

2.1.2 The double helix of DNA is separated using heat during which step of PCR?

• Denaturation

• Annealing

• Extension

• Cloning

This karyotype shows a

• Normal female
• Normal male
• Down
  Down's  Syndrome Female
• Down's Syndrome Male

Tags

NGSS.HS-LS3-1

There would appear to be ____ RFLPs in the gel of a person who is a carrier for Cystic Fibrosis.

• 1

• 2

• 3

• 4