MICROSCOPY - Handling and Focusing

Which of the following is the correct way of handling a microscope?

I - Use only a special lens paper to clean the lenses.

II - Put the microscope on a table with the arm towards you.

III - Keep your microscope covered when not in used.

IV - Carry the microscope with your both hands. Grasp the arm with one hand and place the other hand under the base for support.

V - Do not touch the glass part of the lenses with your fingers.

• I and II

• I, III and IV

• II, III and V

• I, II, III, IV and IV

Which parts should take part in focusing clear images of specimen?

• Objectives

• Course adjustment knob

• Fine adjustment knob

• both coarse and fine adjustment knobs

Where do you place a specimen to observe under a microscope?

• mirror

• eyepiece

• stage clips

• glass slide

If you wanted to have more light, which side of the mirror should be used in a natural light set up?

• Curved, concave side of mirror

• Flat, convex part of mirror

• Any side of the mirror

• all of the answers are correct

You wanted to observe cell walls of onion cells, which is the best objective to be used?

• Scanner

• LPO

• HPO

• OIO

If you need to switch the objectives from scanner to LPO, which mechanical part should be manipulated?

• eyepice

• coarse and fine adjustment knobs

• arm

• revolving nose piece

What is the use of the inclination joint?

• Connects the arm to the base

• makes the microscope bend in reclining position

• condenses the light coming in the microscope

• Both A and B

What is the use of the diaphragm or condenser?

• regulates the light

• captures the light

• refracts the light

• makes the light

Which is the best practice in handling microscope?

• Run while holding the microscope

• Gently slide the microscope on the edge of the table

• Hold the microscope using one hand holding the arm only

• Hold the base and balance the weight of the microscope

Which is the correct sequence in using and focusing?

I - place the specimen on top of the stage and clip it using the stage clips.

II - capture enough light using the mirror. View enough light on the eyepiece.

III - View specimen using the scanner first, followed by the LPO and HPO

IV - Gently adjust the distance of the body tube to the specimen.

V - Use the fine adjustment knob to make clear images of the specimen.

• I, II, III, IV, V

• V, IV, III, II, I

• II, I, IV, III, V

• III, I, IV, V, II