MI Quiz 2.1.1-2.1.3 Review

Single DNA base pair differences between the genomes of individuals are referred to as

• dNTPs.

• SNPs.

• PCRs.

• PTCs.

The next step after extracting and isolating DNA from cheek cells is to

• digest the PCR product of the gene of interest.

• separate the DNA fragments by gel eletrophoresis.

• amplify the gene of interest region of DNA.

• stain the DNA fragments.

Differential digestion by restriction enzyme of DNA is due to

• PCR.

• SNPs.

• PTC.

• gel electrophroesis.

The _________________ is the physical expression of a gene while the _________________ is the gene itself.

• phenotype; genotype

• genotype; phenotype

• gene; genome

• trait; genome

The purpose of gel electrophoresis is to

• digest the gene.

• separate the gene.

• extract the gene.

• amplify the gene.

221 base pairs in the TAS2R38 gene

• is the target of restriction enzymes.

• codes for the entire PTC tasting gene.

• is only found in non-tasters.

• is the sequence of the primers used for PCR.

The DNA sequence shown here is

• a partial sequence of the non-taster allele for the TAS2R38 gene.

• the sequence of the primer used in PCR to amplify the TAS2R38 gene.

• the sequence of the entire TAS2R38 gene.

• a partial sequence of the taster allele for the TAS2R38 gene.

The PTC taste paper strips are used

• to determine the PTC genotype.

• to sequence the TAS2R38 gene.

• to verify the PTC phenotype.

• to isolate the TAS2R38 gene.

The "tasting" allele of the TAS2R38 gene appears to be

• the recessive allele.

• the dominant allele.

• a sex-linked allele.

• a non-functional allele.

The restriction enzyme HaeIII produces

• a blunt end cut in the TAS2R38 gene.

• a sticky end cut in the TAS2R38 gene.

• multiple copies of the TAS2R38 gene.

• a mutation to the TAS2R38 gene.