Genetic Tech Review

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria

What does the acronym CRISPR stand for?

• Controlled Reservative Image Spectacle Palindromic Recounts

• Clustered Regularly-Interspaced Short Palindromic Repeats

Why do bacteria have CRISPR? Check all that apply.

• To be able to remember which viruses they have been infected with

• To be able to edit virus DNA

• To cut apart virus DNA thereby stopping the virus infection

• To be able to edit their own DNA

What is the role of the guide RNA?

• It helps the cell repair the DNA

• ensures that the Cas9 enzyme cuts at the right point in the genome

• Recognizes PAM sequences and opens up the DNA

• makes a cut across both strands of the DNA

How does the guide RNA help the cell know where to cut the DNA?

• Guide RNA helps to find the PAM

• Guide RNA is complementary to the DNA sequence

• Guide RNA always binds to the same sequence of DNA

• Like restriction sites, they are always palindromes

The following sequence is a known GENE MUTATION that geneticists would like to remove. What would be the guide RNA they need to build into a CRISPR-Cas9 enzyme?

TTA CCG

• AAU GGC

• AAT GGC

• TTA CCG

• GCC ATT

What does the CAS9 protein do?

• Prevents viruses from entering bacteria

• Cut RNA

• Cut DNA

• Binds to RNA

Which of the following can be accomplished with CRISPR?

• figure out the role of a protein
• understand genetic diseases
• disable genes
• all three options

Uncontrolled cell division is stopped by...

• tumor suppressor genes
• oncogenes

Normal cell division is regulated by...

• Proto-oncogenes
• oncogenes