Biotechnolgy Review

This is the practice of cutting DNA and separating it by the size of the pieces

• Cloning

• Gel Electrophoresis

• Genetic Engineering

• DNA Fingerprinting

Which kitten is most likely the offspring of this cat?

• 1

• 2

• 3

• 4

Tags

NGSS.HS-LS3-2

NGSS.HS-LS3-1

During gel electrophoresis, the _______ pieces of DNA move farthest down the gel

• lightest

• heaviest

• most mutated

• deadliest

What does transgenic mean?

• An organism that can change its genes at will

• A stem cell that can differentiate into anything

• An organism that has had another species' DNA put in it

• A bacteria that reproduces asexually

Tags

NGSS.HS-LS3-2

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

What does the cas9 protein do?

• Prevents viruses from entering bacteria

• Cut RNA

• Cut DNA

• Binds to RNA

CRISPR can change DNA in humans and animals.  What did scientists look at to help them create CRISPR?

• Sheep cloning
• Palindromic repeats
• Common Cold
• Bacteria fighting off viruses

Some restriction enzymes produce "sticky ends". Sticky ends are . . . 

• single-stranded DNA "overhangs" that can bind to complementary DNA
• smooth cuts that are sticky due to the "goo" left behind by the enzyme
• The two wires (black and red) on a gel box
• The surface of the LB agar plates so that bacteria can grow

What enzyme forms covalent bonds between restriction fragments?

• DNA primase
• DNA helicase
• DNA ligase
• DNA polymerase