EnvMicro_Quiz8

Sampling strategies consider the following except:

• Number of samples

• Type of samples

• Techniques to use

• Person sampling

The following are techniques for soil sampling except:

• Rossi-Cholodny

• Auger

• Grab sampler

• Shovel

Comparing direct and indirect microbial counts:

• Direct counts include dead cells

• Indirect count includes fluorescent stains

• Indirect count uses selective media

• Direct counts are less accurate

Enumeration of bacterial cells can be achieved with the following except:

• Spread plate

• Flow cytometry

• DGGE

• Pour plate

The following technique allows for identification of species:

• RAPD-PCR

• RT-PCR

• Metagenomics

• 16S-rRNA

The best technique to detect for change in microbial communities over a period of time:

• RT-PCR

• RAPD-PCR

• Metagenomics

• DGGE

The MPN method can be used for:

• Soil samples

• Water samples

• Food samples

• All

Fungal cultures can be identified via:

• Melanized hyphae

• RT-PCR

• 16S-rRNA sequencing

• None

Mycoviruses:

• Causes hypervirulence

• Is found in all fungi

• Causes hypovirulence

• All

Which technique allows for staining differentiation between bacteria with different genes?

• Gram staining

• Flow cytometry

• PCR-FISH

• Real-time PCR