PCR

What happens during the Denaturation step of PCR?

• The double-stranded DNA melts open to form two single strands.

• The DNA strands are synthesized into a new double helix.

• The DNA is amplified by adding more nucleotides.

• The DNA is cooled to allow for primer binding.

Tags

NGSS.HS-PS1-2

What is the second step in PCR?

• Denaturation

• Extension

• Primer annealing

• DNA replication

What is the final product of PCR?

• A single copy of the target DNA sequence.

• Millions of copies of the target DNA sequence.

• A mixture of DNA fragments of varying lengths.

• No product is generated during PCR.

How many cycles are typically performed in PCR?

• 10-15 cycles

• 20-25 cycles

• 25-35 cycles

• 40-50 cycles

What happens during the Extension step of PCR?

• DNA polymerase adds nucleotides to the 3' end of the primer, synthesizing a new DNA strand.

• DNA ligase seals the nicks in the DNA backbone.

• Primers are removed from the DNA template.

• DNA helicase unwinds the DNA double helix.

What enzyme is primarily used in PCR for DNA synthesis?

• RNA polymerase

• DNA ligase

• DNA polymerase

• Reverse transcriptase

What is the first step in PCR?

• Denaturation

• Annealing

• Extension

• Replication

What is the purpose of PCR?

• To produce millions of copies of a specific region of DNA.

• To sequence the entire genome of an organism.

• To analyze protein structures in a sample.

• To isolate RNA from a DNA sample.

Does PCR require a primer?

• Yes.

• No.

• Only in some cases.

• It depends on the type of PCR.

What is PCR?

• A technique used to amplify specific segments of DNA.

• A method for sequencing RNA.

• A process for protein synthesis.

• A technique for cloning entire organisms.