What do you already know about bioinformatics?

Which of these databases contains nucleotide sequences?

• GenBank

• OMIM

• PubMed

• UniProt

Which of these databases contains protein sequences?

• GenBank

• OMIM

• PubMed

• UniProt

Which of these databases contains scientific publications?

• GenBank

• OMIM

• PubMed

• UniProt

UniProt stores

• Curated and predicted proteins

• Predicted proteins only

• Curated proteins only

• Protein functions only

SwissProt stores

• Curated and predicted proteins

• Predicted proteins only

• Curated proteins only

• Protein functions only

The NCBI RefSeq database

• contains sequences of wildtype and mutated genes

• only sequences of wildtype genes

• only sequences of mutated genes

FastA is a

• fixed sequence format

• fixed vocabulary for function annotation

• tool to compare sequence similarity

• database for functional annotation

BLAST can be used to

• look for metabolic maps

• find similarities in gene expression

• look for sequence homology

• get EC numbers of enzymes

Significant BLAST results always have

• a very high E-value

• a very low E-value

If I am looking at the same gene or protein in different species, I am looking at

• ortholog[ue]s

• homolog[ue]s

• paralog[ue]s

• catalog[ue]s