Protoplast: Introduction & Isolation

Cell without a nucleus

Cell without a Cell wall

Cell without a Plasma membrane

Cell without a genetic material

Selection of Explant

Viability testing for protoplast

Isolation of protoplast

Preparation of culture media

To degrade proteins

To degrade cellulose

To degrade pectin

To degrade hemicellulose

Protoplast hybrids

Protoplast fusion

Somatic hybridization

Somatic hybrids

Carlson et al

Hanstein

Klercker

Cocking

Hanstein

Klercker

Cocking

Tkebe et al

Klercher

Tkebe et al

Cocking

Both A & B

protoplasts released are few in number.

protoplasts are damaged

Viability of protoplasts is high

All of the above

Macerozyme

Rhozyme HP 150

Cellulase

Pectiolose

low starch content generally yields better results

high starch content generally yields better results

low sucrose content generally yields better results

low sucrose content generally yields better results