Enzyme immobilisation

The immobilization technique involving physical method is

• covalent bond formation dependent

• non-covalent bond formation dependent

• both (a) and (b)

• ionic bond formation dependent

Which of the following is the commonly employed adsorbents?

• Calcium carbonate

• Alumina

• Celluloses

• All of these

Which of the following is not a physical method of immobilization?

• Adsorption

• Entrapment

• covalent bond formation

• None of these

Which of the following is considered as a disadvantage to the adsorption method of immobilization?

• It is possible to separate and purify the enzymes while being immobilized

• The enzymes are not usually deactivated by adsorption

• The adsorption is a reversible process

• State of immobilization is very sensitive to solution pH, ionic strength and temperature

The immobilized technique involving chemical method is

• covalent bond formation dependent

• non-covalent bond formation dependent

• both (a) and (b)

• ionic bond formation dependent

Enzyme immobilisation enhances which of the following properties of an enzyme

• proteinous nature

• specificity

• thermal stability

• catalytic ability

In a reaction system that involves immobilised enzymes, …………………………

• both enzymes and substrate can flow around

• only enzymes but not substrates can flow around

• only substrates but not enzymes can flow around

• neither substrate not enzymes can flow around

Immobilised enzymes have higher thermal stability compared to free enzymes. Which of the following statements is an inaccurate interpretation of this?

• Immobilised enzymes have higher optimum temperature

• Immobilised enzymes can keep on catalysing reaction even when their free counterparts have become denatured by high temperature

• No matter how high temperature gets, immobilsed enzymes will keep on catalysing reaction

• immobilsed enzymes have a wider range of temperature within which they can act, compared to free enzymes

In using immobilized enzymes to produce lactose free milk, the enzymes will be found in which labelled part of the diagram?

• A only

• A and B

• B and C

• B only

immobilised enzymes are characterised by ………………thermal stability and ……………….reactivity

• higher, higher

• higher, lower

• lower, lower

• lower, higher