Gel electrophoresis

Which type of gel is commonly used for separating proteins in electrophoresis?

• Agarose gel

• Polyacrylamide gel

• Cellulose acetate gel

• Starch gel

In gel electrophoresis, what factor primarily affects the migration speed of DNA fragments?

• Buffer composition

• Temperature

• Voltage applied

• Size of the gel pores

what is the purpose of running gel electrophoresis?

• Purify molecules

• identify and purify

• analyze, identify and purity

• Analyze and purity

SDS-PAGE separates proteins based on:

• Charge

• Molecular weight

• Base pairs

• Molecular weight and charge

What is the role of the loading dye in gel electrophoresis?

• To enhance the conductivity of the gel

• To visualize the DNA fragments

• To increase the pH of the gel

• To track the progress of the electrophoresis

Which of the following is a common buffer used in gel electrophoresis?

• Tris-Borate-EDTA (TBE)

• Phosphate Buffered Saline (PBS)

• Acetic Acid

• Sodium Chloride (NaCl)

What is the purpose of using a DNA ladder in gel electrophoresis?

• To increase the voltage

• To serve as a size reference

• To stabilize the gel

• To enhance the visibility of bands

What is the primary purpose of using a gel in electrophoresis?

• To separate molecules based on size

• To enhance the visibility of proteins

• To increase the temperature of the sample

• To stabilize the electric field

Which of the following factors can affect the resolution of bands in gel electrophoresis?

• All of the above

• Voltage applied

• Type of buffer used

• Gel concentration

What is the significance of using a control sample in gel electrophoresis?

• To ensure the gel is functioning properly

• To reduce the amount of sample needed

• To compare the results with experimental samples

• To increase the speed of electrophoresis