1.3.1 DNA Detectives

In Part I DNA Digestion with Restriction Enzymes

If I'm going to do a 30-60 minute restriction digestion, what temp should my water bath be?

• 37 degrees C

• 45 degrees C

• 37 degrees F

• 45 degrees F

In Part I DNA Digestion with Restriction Enzymes

How many tubes does your team need to label for this lab?

• 1

• 2

• 3

• 4

In Part I DNA Digestion with Restriction Enzymes

What do you add to your tubes RIGHT BEFORE freezing them?

• SYBRSafe

• 10x gel loading dye (solution)

• enzyme

• DNA

In the gel making video, when making a 30 ml gel, what mass of agarose powder should be added to the buffer to make the gel?

• 0.24g

• 0.48g

• .008g

• 24g

In Part I DNA Digestion with Restriction Enzymes

How much Enzyme 1 will you need TOTAL for this lab (this includes for both Missing Person 1 and Missing Person 2)

• 15 microliters

• 25 microliters

• 30 microliters

• 45 microliters

What's the purpose of adding the restriction enzymes to your sample? (Remember what restriction enzymes do from the Bozeman Video)

• they'll separate the DNA bases

• they'll copy the DNA

• they'll cut the DNA

• they'll make the DNA glow

From your gel making video notes, what role will SYBR Safe play in this lab?

• It will make the DNA strands move

• It will make the DNA strands separate

• It will copy the DNA strands

• It will make the DNA strands visible

From the gel making video, why do we wrap the gel in aluminum foil after making it?

• to prevent light from damaging the stain

• to keep it moist

• to allow the DNA strands to be copied

• to hide it from other teams

What's the purpose of gel electrophoresis?

(Check your Bozeman Video notes)

• to make the DNA visible

• to separate the pieces of DNA

• to tag the DNA pieces with color

• to copy the pieces of DNA

Part 2 Gel Electrophoresis of Restriction Enzymes

When loading your gel, what should go into lane 6?

(MP=Missing Person, E=Enzyme)

• MP 1 cut with E1

• MP 1 cut with E2

• MP 2 cut with E1

• MP 2 cut with E2