PBS 2.2.6 Quiz Review (Quizizz)

An accumulation or build-up of a fatty substance (cholesterol) on the walls of arteries is called

• hypercholesterolemia.

• RFLP.

• plaque.

• electrophoresis.

• restriction.

The type of gel being used for electrophoresis is called

• agarose.

• RFLP.

• Edvotek.

• gelatin.

• FH.

The gene that causes familial hypercholesterolemia is

• autosomal dominant.

• on chromosome 17.

• caused by a defect in the LDL receptor.

• autosomal recessive.

• caused by a defect in how the liver processes HDL.

Tags

NGSS.HS-LS3-2

People with familial hypercholesterolemia (FH)

• have high levels of cholesterol in their blood.

• have high levels of HDL in their blood.

• have high levels of LDL in their blood.

• can be fine with just diet and exercise.

• usually require taking medication while watching their diet and following an exercise routine.

Tags

NGSS.HS-LS3-2

Differences in the sequence of DNA between those that have familial hypercholesterolemia (FH) and those that don't (wild-type)

• are due to polymorphisms or mutations.

• can be detected by using restriction enzymes.

• can be visualized on a gel because these differences would result from using different restriction enzymes.

• can be visualized on a gel because these differences would result in DNA of different lengths.

• are usually found in multiple chromosomes.

Tags

NGSS.HS-LS3-2

In order to withdraw a specific volume or quantity of liquid sample using a micropipette, you depress the plunger

• a tiny bit.

• all the way down.

• multiple times, up and down.

• to the first point of resistance.

• very fast.

In order to expel (release) all of the liquid sample using a micropipette, you

• twist the volume adjuster clockwise.

• depress the plunger all the way down.

• twist the volume adjuster counterclockwise.

• depress the blue pipet tip release lever.

• depress the plunger to the first point of resistance.

In order to visual differences in DNA genotypes (like who is FF, who is Ff, who is ff), we use RFLP. Before RFLP, we need to

• use PCR to amplify the DNA.

• use gel electrophoresis to separate the DNA.

• use restriction enzymes to cut the DNA.

• use micropipettes to dispense DNA samples in the gel.

• use SybrSafe to stain the DNA.

To make a 2-group batch of agarose gel, you need

• 60 mL of agarose powder.

• 0.23 g of agarose powder.

• 30 mL of agarose powder.

• 6 ul of agarose powder.

• 0.46 g of agarose powder.

In order to properly run the gel, you need to set the power source to

• 20 V.

• 25 ul.

• 130 V.

• 30 mL.

• 30 V.