Paper 4 Figure 3

Based on the figure, what is the role of ppkA?

•  acts as a kinase

• autophosylates itself

•  phosphorylates an essential T6SS apparatus component

• all of the above

Can dueling occur between clonemates?

• Yes, it does occur between P. aeruginosa clonemates

• No, that’s silly because it would be illogical fitness-wise (energy cost is too high) to attack your own clonemates even if they have the immunity proteins to the toxins of the T6SS

In this signaling cascade Fha1 is:

• The protein that phosphorylates

• The protein that dephosphorylates PppA

• The protein that autodephosphorylates

• The protein that gets phosphorylated by PpkA

Upon the inactivation of pppA, which of these does NOT occur:

• T6SS are generally localized to a certain spot in the cell

• T6SS do not get disassembled

• T6SS keep firing in the absence of a signal since their ability to sense the cessation of a signal is lost

• T6SS increase dueling behavior due to an increase in T6SS activity

What is an ATP-binding cassette transporter?

• Type of passive transporters that allow transfer of molecules through the cell membrane 

• Type of primary active transporters that have membrane bound proteins that perform influx or efflux of cells

• Type of facilitated diffusion that allows for the transfer of molecules through a cell membrane with the help of a membrane proteins.

• Type of secondary active transporter that allow molecules to move down an electrochemical gradient

What is the overall goal of the experiment in Figure 3C?

• To determine the T6SS activity and dueling behavior of P. aeruginosa with the inactivation of TagT.

• To determine the T6SS activity and dueling behavior of P. aeruginosa with the inactivation of pppA.

• To determine the T6SS activity and dueling behavior of P. aeruginosa with the inactivation of kinase.

• o determine the T6SS activity and dueling behavior of P. aeruginosa with the inactivation of ppkA.

What are the results in Figure 3C?

• The tagT knockout gene on P. aeruginosa lost their dueling activity and their T6SS activity.

• The tagT knockout gene on P. aeruginosa lost their dueling activity and had T6SS activity present.

• The tagT knockout gene on P. aeruginosa had dueling activity present and T6SS activity present.

• None of the above.

Main Takeaway from Figure 3 was that:

• T6SS Dueling is not impacted by the T6SS regulatory pathway

• T6SS Dueling is only impacted by mutation of a single really important protein in the regulation pathway

• T6SS Dueling is impacted in different ways by inactivating certain key proteins of the T6SS regulatory pathway

• T6SS Dueling does not occur in P. aeruginosa